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Pediatrics Hematology/Oncology Research Assistant

20001977 Requisition #
The Stead Family Department of Pediatrics, Division of Hematology/Oncology is seeking a research assistant to be responsible for maintaining the laboratory and performing research related activities within the Stead Family Department of Pediatrics, Division of Hematology/Oncology.  This position will conduct experiments, collect data, and analyze results.  This is a specified term position.

It is expected that as the research assistant gains experience with laboratory techniques and procedures, they will take on increased autonomy and oversight in the lab.  They will also gradually increase the scientific level of their duties by adding experimental design and data analysis as well as preparation of materials for abstracts/reports/grant applications and manuscripts.  Position responsibilities include:

• Perform research activities related to the analysis of coagulation including factor VIII and von Willebrand factor protein and activity measurement and measurement of immune responses against the protein.
• Perform research on other specified topics and prepare bibliographies and abstracts according to instructions.
• As individual skills develop, analyze literature to develop hypothesis for discussion with other researchers or principal investigator.
• Perform cloning; DNA, RNA and protein based studies of gene expression; construction and production of recombinant AAV, lentiviral, and adenoviral vectors and non-viral vectors.
• Assist with measurement of cellular and humoral immune responses against proteins using ELISA and Bethesda assay.
• Assist with in vitro transposition assays using viral and non-viral vectors.
• Assist with immunohistochemistry and immunofluorescent studies.
• Assist with in vivo and in vitro gene transfer in mouse model including but not limited to tail vein injection, hydrodynamic tail vein injections, tracheal intubation, retro-orbital bleeding, thrombosis studies, anesthesia, ferric chloride injury, tissue harvest, behavior testing, and bioluminescent imaging.
• Perform gene expression studies on cells and tissues, operate light microscopes and acquire microscopic imaging data including confocal.
• Handling and care of mice; mouse colony breeding and management to include mouse tail injections. Maintain mouse colony records.
• Read and understand protocols.
• Troubleshoot and analyze protocols; propose modifications to other researchers or principal investigator.
• Perform complex techniques as outlined in research protocols and test and evaluate current procedures.
• Perform experimental procedures assuring fidelity to protocols; record and consolidate data as directed by Principal Investigator (PI).
• Track progress/success of research project.
• Present results at lab meetings; develop and monitor quality controls.
• Assist with gene expression analysis including mRNA preparation, cDNA library preparation, gene chip and sequencing methods, and bioinformatic analysis.
• Assist with bioinformatic computer analysis using Sequencher, DNAStar Lasergene, Adobe CS4 Suite, GSEA, and Ingenuity Pathway Analysis. 
• Prepare materials for reports to be written by faculty and staff on the research project.
• Collect, track, and perform data entry for reports.
• Perform a variety of routine tasks related to the recruitment, enrollment and monitoring of research subjects or to the research environment (lab, clinic, field, classroom).
• Obtain subject consent and assist with IRB submissions or equivalent protocol, if applicable.
• Adhere to safety and compliance guidelines.
• Calibrate various laboratory equipment and operate microscopes and centrifuges
• Assist in the preparation, calibration, and maintenance of laboratory and facility equipment.
• Assist with procurement of supplies and materials.
• May initiate purchasing requests, monitor accounts/expenditures; reconcile statements.

Percent of Time: 100%
Pay Grade: 3A
Pay Structure A: https://hr.uiowa.edu/professional-pay/compensation-plan-structure-a-FY18

Benefits Highlights:
• Regular salaried position located in Iowa City, Iowa
• Fringe benefit package including paid vacation; sick leave; health, dental, life and disability insurance options; and generous employer contributions into retirement plans.
• For more information about Why Iowa?, click here
Required Qualifications
• A  Bachelor's degree in a natural or health science field or discipline, or an equivalent combination of education and experience. 
• Demonstrated experience with analysis and management programs such as DNAStar Lasergene.
• Demonstrated excellent written and verbal communication and interpersonal skills.
• Minimum of 3 months experience of immunofluorescence and confocal imaging of hepatocytes and neurons.

Highly Desired Qualifications
• Demonstrated experience in viral and non-viral vector production and in vivo and in vitro gene transfer techniques.
• Demonstrated experience in factor VIII and von Willebrand factor ELISA measurement.
• Demonstrated experience in mouse behavior testing.
• Demonstrated experience in tissue sectioning and immunohistochemistry.
• One year experience caring for and handling mice to include mouse colony maintenance and management is highly desired.

Please attach a resume and cover letter as part of the application process.  Job openings are posted for a minimum of 14 calendar days.  Applications will be accepted until 11:59 PM on April 22, 2020.  This job may be removed from posting and filled any time after the minimum posting period has ended. For questions or additional information, please contact Pediatrics Human Resources at pedsuichildrenshr@healthcare.uiowa.edu. 

This position is not eligible for University sponsorship for employment authorization. Successful candidates will be subject to a credential and criminal background check.

Equal opportunity/affirmative action employer

The University of Iowa is an equal opportunity/affirmative action employer. All qualified applicants are encouraged to apply and will receive consideration for employment free from discrimination on the basis of race, creed, color, national origin, age, sex, pregnancy, sexual orientation, gender identity, genetic information, religion, associational preference, status as a qualified individual with a disability, or status as a protected veteran.

Persons with disabilities who need assistance or accommodations with the application or interview process may contact University Human Resources/Faculty and Staff Disability Services, (319) 335-2660 or fsds@uiowa.edu.  For jobs in UI Health care, please contact UI Health care Leave & Disability Administration at 319-356-7543.

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